Analyzing the relationship of Leishmania chagasi and complement resistance through protein-protein interactions, genetic complementation, and innovative techniques
نویسندگان
چکیده
Serial passage of axenically-cultured Leishmania chagasi promastigotes results in a progressive diminution in resistance to complement mediated lysis (CML), whereas high CML resistance is seen in infectious metacyclic promastigotes from the sandfly vector as well as metacyclic-like promastigotes within low passage cultures at stationary growth phase. As previously reported [94], in a screen seeking to identify novel genes involved in CMLresistance, (i) a genomic cosmid library derived from DNA of CML-resistant L. chagasi promastigotes was transfected into high passage (constitutively CML-sensitive) L. chagasi promastigotes, (ii) transformants were screened for acquisition of CML-resistance, (iii) multiple cosmid-transfectants exhibited partial CML resistance, and (iv) the sequence for one
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Genetic complementation to identify DNA elements that influence complement resistance in Leishmania chagasi.
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